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Diagram of air–liquid-interface platform for dust exposure. ( A ) Stepwise experimental timeline of the decellularized plant-based ALI model and dust exposure. ( B ) 3D design of the platform, which provides an enclosed chamber for accommodating spinach leaves. ( C ) The macroscopic image of the recellularized spinach integrated into the air–liquid-interface platform. Live/Dead fluorescence images (Calcein-AM = <t>green,</t> <t>EthD-1</t> = red) of recellularized leaves (Left and right panels correspond to images taken from different focal planes representing the apical epithelial surface and the vascular endothelial layer, respectively). ( D ) Quantitative cell viability of BEAS-2B and HUVECs analyzed using ImageJ software (NIH, USA).
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Diagram of air–liquid-interface platform for dust exposure. ( A ) Stepwise experimental timeline of the decellularized plant-based ALI model and dust exposure. ( B ) 3D design of the platform, which provides an enclosed chamber for accommodating spinach leaves. ( C ) The macroscopic image of the recellularized spinach integrated into the air–liquid-interface platform. Live/Dead fluorescence images (Calcein-AM = <t>green,</t> <t>EthD-1</t> = red) of recellularized leaves (Left and right panels correspond to images taken from different focal planes representing the apical epithelial surface and the vascular endothelial layer, respectively). ( D ) Quantitative cell viability of BEAS-2B and HUVECs analyzed using ImageJ software (NIH, USA).
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Diagram of air–liquid-interface platform for dust exposure. ( A ) Stepwise experimental timeline of the decellularized plant-based ALI model and dust exposure. ( B ) 3D design of the platform, which provides an enclosed chamber for accommodating spinach leaves. ( C ) The macroscopic image of the recellularized spinach integrated into the air–liquid-interface platform. Live/Dead fluorescence images (Calcein-AM = <t>green,</t> <t>EthD-1</t> = red) of recellularized leaves (Left and right panels correspond to images taken from different focal planes representing the apical epithelial surface and the vascular endothelial layer, respectively). ( D ) Quantitative cell viability of BEAS-2B and HUVECs analyzed using ImageJ software (NIH, USA).
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Diagram of air–liquid-interface platform for dust exposure. ( A ) Stepwise experimental timeline of the decellularized plant-based ALI model and dust exposure. ( B ) 3D design of the platform, which provides an enclosed chamber for accommodating spinach leaves. ( C ) The macroscopic image of the recellularized spinach integrated into the air–liquid-interface platform. Live/Dead fluorescence images (Calcein-AM = <t>green,</t> <t>EthD-1</t> = red) of recellularized leaves (Left and right panels correspond to images taken from different focal planes representing the apical epithelial surface and the vascular endothelial layer, respectively). ( D ) Quantitative cell viability of BEAS-2B and HUVECs analyzed using ImageJ software (NIH, USA).
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Diagram of air–liquid-interface platform for dust exposure. ( A ) Stepwise experimental timeline of the decellularized plant-based ALI model and dust exposure. ( B ) 3D design of the platform, which provides an enclosed chamber for accommodating spinach leaves. ( C ) The macroscopic image of the recellularized spinach integrated into the air–liquid-interface platform. Live/Dead fluorescence images (Calcein-AM = <t>green,</t> <t>EthD-1</t> = red) of recellularized leaves (Left and right panels correspond to images taken from different focal planes representing the apical epithelial surface and the vascular endothelial layer, respectively). ( D ) Quantitative cell viability of BEAS-2B and HUVECs analyzed using ImageJ software (NIH, USA).
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Diagram of air–liquid-interface platform for dust exposure. ( A ) Stepwise experimental timeline of the decellularized plant-based ALI model and dust exposure. ( B ) 3D design of the platform, which provides an enclosed chamber for accommodating spinach leaves. ( C ) The macroscopic image of the recellularized spinach integrated into the air–liquid-interface platform. Live/Dead fluorescence images (Calcein-AM = <t>green,</t> <t>EthD-1</t> = red) of recellularized leaves (Left and right panels correspond to images taken from different focal planes representing the apical epithelial surface and the vascular endothelial layer, respectively). ( D ) Quantitative cell viability of BEAS-2B and HUVECs analyzed using ImageJ software (NIH, USA).
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Diagram of air–liquid-interface platform for dust exposure. ( A ) Stepwise experimental timeline of the decellularized plant-based ALI model and dust exposure. ( B ) 3D design of the platform, which provides an enclosed chamber for accommodating spinach leaves. ( C ) The macroscopic image of the recellularized spinach integrated into the air–liquid-interface platform. Live/Dead fluorescence images (Calcein-AM = <t>green,</t> <t>EthD-1</t> = red) of recellularized leaves (Left and right panels correspond to images taken from different focal planes representing the apical epithelial surface and the vascular endothelial layer, respectively). ( D ) Quantitative cell viability of BEAS-2B and HUVECs analyzed using ImageJ software (NIH, USA).
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Diagram of air–liquid-interface platform for dust exposure. ( A ) Stepwise experimental timeline of the decellularized plant-based ALI model and dust exposure. ( B ) 3D design of the platform, which provides an enclosed chamber for accommodating spinach leaves. ( C ) The macroscopic image of the recellularized spinach integrated into the air–liquid-interface platform. Live/Dead fluorescence images (Calcein-AM = <t>green,</t> <t>EthD-1</t> = red) of recellularized leaves (Left and right panels correspond to images taken from different focal planes representing the apical epithelial surface and the vascular endothelial layer, respectively). ( D ) Quantitative cell viability of BEAS-2B and HUVECs analyzed using ImageJ software (NIH, USA).
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Diagram of air–liquid-interface platform for dust exposure. ( A ) Stepwise experimental timeline of the decellularized plant-based ALI model and dust exposure. ( B ) 3D design of the platform, which provides an enclosed chamber for accommodating spinach leaves. ( C ) The macroscopic image of the recellularized spinach integrated into the air–liquid-interface platform. Live/Dead fluorescence images (Calcein-AM = green, EthD-1 = red) of recellularized leaves (Left and right panels correspond to images taken from different focal planes representing the apical epithelial surface and the vascular endothelial layer, respectively). ( D ) Quantitative cell viability of BEAS-2B and HUVECs analyzed using ImageJ software (NIH, USA).

Journal: Scientific Reports

Article Title: A perfusable decellularized plant-based air-liquid-interface-on-a-chip for investigating inorganic dust aerosol exposure

doi: 10.1038/s41598-025-33930-7

Figure Lengend Snippet: Diagram of air–liquid-interface platform for dust exposure. ( A ) Stepwise experimental timeline of the decellularized plant-based ALI model and dust exposure. ( B ) 3D design of the platform, which provides an enclosed chamber for accommodating spinach leaves. ( C ) The macroscopic image of the recellularized spinach integrated into the air–liquid-interface platform. Live/Dead fluorescence images (Calcein-AM = green, EthD-1 = red) of recellularized leaves (Left and right panels correspond to images taken from different focal planes representing the apical epithelial surface and the vascular endothelial layer, respectively). ( D ) Quantitative cell viability of BEAS-2B and HUVECs analyzed using ImageJ software (NIH, USA).

Article Snippet: Cell viability within the recellularized spinach scaffolds was evaluated using a Calcein-AM/Ethidium homodimer-1 (EthD-1) Live/Dead Viability/Cytotoxicity Kit (Biotium, USA) following the manufacturer’s protocol.

Techniques: Fluorescence, Software